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KMID : 0364219900330010103
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Korean Journal of Zoology
1990 Volume.33 No. 1 p.103 ~ p.107
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Structural Characterization of an IgM-like Immunoglobulin in the Serum of Swamp Eel, Monopterus albus
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Son Young-Jong
Yi Jung-Woo
Chang Chung-Soon
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Abstract
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lgM-like immunoglobulin was purified from the immune serum of M albus which immunized with bovine Serum albumin(BSA) as an antigen(Ag) and characterized. The Ag-specific antibody activity of the immune serum was increased after the immunization. The purified lgM-like immunoglobulin had a tetrameric structure which had a molecular weight of 800 kD and the monomer of IgM-like Ig had a mass of 199 kD which was composed of two heavy chains (Mol. wt. 70 kD) and iwo light chains (Mol. wt. 29.5 kD). The IgM-like Ig showed hemaggluti nating activity to mammalian RBC slightly.
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KEYWORD
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fish , Monopterus albus , Immunoglobulin , Milecular stucture
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